Test Name
PML/RARA qRT PCR, short and long form, send out
Test Code
M PML RARA*
Synonyms
PML RARA t(15;17) short form, PML RARA t(15;17) long form
Associations
Acute promyelocytic leukemia (APL), FAB M3;
ATRA
CPT Codes
81315, G0452
Turnaround Time
10 days
Specimen Requirements
5 mL (Min.1 mL) whole blood; 3 mL (Min.1 mL) bone marrow; EDTA only
Specimen Stability
Stable at 2-8°C; Specimen must be received by MPLN within 48 hours of collection. Stability (collection to initiation of testing): Ambient: 1 hour; Refrigerated: 48 hours
Storage & Handling
Specimens must be received within 48 hours of collection due to lability of RNA. Ship peripheral blood and bone marrow with cool/refrigerated pack (Do not allow cool/refrigerated pack to directly contact sample) Please call before shipping on Friday for
Causes for Rejections
Specimen >48 hours old; Frozen Specimen; Specimen clotted; Stored or shipped at incorrect temperature; Incorrect anticoagulant; Insufficient specimen volume; Severely hemolyzed specimen
Description
Utilized for the determination of baseline PML/RARA fusion gene transcript levels and monitoring of same for molecular evidence of minimal residual disease (MRD) or molecular remission in patients with AML-M3 (APL) after induction and consolidation therap
References
- Gabert J et al. (2003). Standardization and quality control studies of real time quantitative reverse transcriptase polymerase chain reaction of fusion gene transcripts for residual detection in leukemia-A Europe against cancer program. Leukemia. 17:2318-2357.
- Reiter et al. (2004). Pathogenesis, diagnosis and monitoring of residual disease in acute promyelocytic leukaemia. Acta Haematologica. 112:55
- Sanz MA. (2006). Treatment of acute promyelocytic leukemia. Hematology. 147-155.
