Test Name
C MYC 8q24 Rearrangement, paraffin
Test Code
FP CMYC
Synonyms
MYC, 8q24 translocation
Associations
Lymphoma, Burkitt, Diffuse large B-cell, Solid tumors, Breast and prostate cancer
CPT Codes
88377, 88271
Turnaround Time
5
Specimen Requirements
FFPE tissue is acceptable for FISH analysis. Preferred fixative is 10% neutral buffered formalin. Tissues preserved in B5 fixative or decalcified are usually not suitable for FISH. Tumor sections cut 3-5 µm thick and mounted on positively charged organosilane coated (silanized) slides work well. Request several unstained sections (two for each probe) and one H&E stained slide
Specimen Stability
Stable indefinitely when stored at 20°C to 25°C
Storage & Handling
4°C to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.
Causes for Rejections
Improper fixative; Tissue not fixed; Insufficient tissue; Specimen exposed to extreme temperature
Description
Translocations of chromosome 8 are common aberrations in the B-cell non-Hodgkin lymphoma group. The presence of the typical t(8;14) or the rare variants t(8;22) and t(2;8) has been confirmed in all cases of Burkitt lymphoma and in some cases of Burkitt-li
References
- Pienkowska-Grela et al. (2005). Cancer Genet Cytogenet. 156(2):114.
