Test Name
IGH/BCL2 t(14;18), paraffin
Test Code
FP BCL2
Synonyms
IGH/BCL2; t(14;18)(q32;q21)
Associations
B-cell lymphoma (BCL), Non-Hodgkin lymphoma (NHL), Follicular lymphoma (FCL)
CPT Codes
88377, 88271
Turnaround Time
5
Specimen Requirements
FFPE tissue is acceptable for FISH analysis. Preferred fixative is 10% neutral buffered formalin. Tissues preserved in B5 fixative or decalcified are usually not suitable for FISH. Tumor sections cut 3-5 µm thick and mounted on positively charged organosilane coated (silanized) slides work well. Request several unstained sections (two for each probe) and one H&E stained slide
Specimen Stability
Stable indefinitely when stored at 20°C to 25°C
Storage & Handling
4°C to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.
Causes for Rejections
Improper fixative; Tissue not fixed; Insufficient tissue; Specimen exposed to extreme temperature
Description
BCL2 blocks apoptosis and maintains long-term immune responsiveness including B-cell memory. The most frequent non-random chromosome rearrangement in non-Hodgkin lymphoma (NHL) of B-cell type is t(14;18)(q32;q21), which is found in approximately 50–85% of
References
- Noreiga M et al. (2004). Blood Cells, Molecules and Diseases. Volume 32, Issue 1. 232-239.
- Vaandrager J et al. (2000). Genes Chrom Cancer. 25:85-94.
