Test Name
Flow Cytometry Technical Component Only
Test Code
FLOW TC
Synonyms
Immunophenotyping, myeloid / lymphoid markers
Associations
Acute myeloid leukemia, Acute lymphoblastic leukemia, Chronic lymphocytic leukemia, Non-Hodgkin lymphoma
Reflex Testing
Fish, Cytogenetics, Molecular
Turnaround Time
1
Specimen Requirements
Whole blood: 4 mL (min 1 mL) in sodium heparin or EDTA; Bone marrow aspirate: 2 mL (min 0.5 mL) in sodium heparin or EDTA; Bone marrow core biopsy: 2 cm (min 1 cm) in sterile container with 2-4 mL MPLN RPMI transport media; Fresh tissue: 5 mm^3 in sterile container with 2-4 mL MPLN RPMI transport media; Fine needle aspirate: 4 mL (min 0.5 mL) in sterile container with 2-4 mL MPLN RPMI transport media; Fluids (cerebrospinal, pleural, synovial, pericardial, etc): 4 mL (min 0.5 mL) in sterile container with 2-4 mL MPLN RPMI transport media; Bronchoalveolar lavage: 10 mL (min 5 mL) in sterile container with 2-4 mL MPLN RPMI transport media. If RPMI media or MPLN transport media is not available, send in sterile saline.
Specimen Stability
Specimen stable for 48 hours at 4°C
Storage & Handling
Ship with ice pack. Protect from extreme temperature. Separate ice pack from specimen.
Causes for Rejections
Specimens stored at incorrect temperature; Non-viable specimens; Specimens in inappropriate anticoagulant; Too few cells; Hemolysis; Specimen clotted
Description
Immunophenotyping, myeloid / lymphoid markers by Flow cytometry
References
- van de Loosdrecht AA, 2009, Standardization of flow cytometry in myelodysplastic syndromes: report from the first European LeukemiaNet working conference on flow cytometry in myelodysplastic syndromes, Haematologica Aug 2009, 94 (8) 1124-1134
- Craig and Foon. (2008). Flow cytometric immunophenotyping for hematologic neoplasms. Blood 111, No. 8:3941-3967.
- Bethesda International Consensus Conference on Flow Cytometric Immunophenotyping of Hematopoietic Neoplasia. (2006).
- Davis BH et al. (2006). Bethesda International Consensus Recommendations on the Flow Cytometric Immunophenotypic Analysis of Hematolymphoid Neoplasia: Medical Indications (submitted for publication, Cytometry)
- Nguyen et al. (2003). Flow Cytometry in Hematopathology. Humana Press.
- Shapiro H (2003). Practical Flow Cytometry 4th edition. Wiley-Liss.
- Wells et al. (2003). Myeloid and monocytic dyspoiesis as determined by flow cytometric scoring in myelodysplastic syndrome correlates with IPSS and with outcome after hematopoietic stem cell transplantation. Blood 102(1):394-403.
- Foucar. (2001). Bone Marrow Pathology 2nd edition. ASCP Press.
