Test Name
Trisomy 5, 9, 15
Test Code
F T5T9T15
Synonyms
+5p/+9/+15
Associations
Multiple Myeloma
CPT Codes
88374
Turnaround Time
5
Specimen Requirements
5mL peripheral blood in sodium heparin 3mL bone marrow in sodium heparin Fixed cytogenetically prepared cells in sterile centrifuge tube with pellet visible in 3:1, Methanol:Acetic Acid
Specimen Stability
Blood and bone marrow = 4°C to 25°C, specimens are stable up to 72 hours Fixed cell pellets are stable for years when stored at -28°C to -15°C
Storage & Handling
4°C to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.
Causes for Rejections
Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells
Description
Aneuploidy is a frequent finding in plasma cell myeloma. Approximately 60-70% of myeloma cases have been reported to have hyperdiploid clones. About 10-20% have been reported to have pseudodiploid clones, and 10-30% have been reported to have hypodiploid
References
- Fonseca et al. (2004). Genetics and cytogenetics of multiple myeloma: A workshop report. Cancer Res. 64:1546. Hideshima et al. (2004). Advances in biology of multiple myeloma: clinical applications. Blood. 104:607.
- Pratt G. (2002). Molecular aspects of multiple myeloma. J Clin Pathol: Mol Pathol. 55:273.
- Schmidt-Wolf et al. (2006). Cancer Genetics and Cytogenetics. 167:20–25.
