Test Name
Plasma Cell Neoplasms FISH panel
Test Code
F MM
Synonyms
1p-, 1q+, 5,+9, t(11;14), 13q-, +15, 17p-, reflex t(4;14) / t(14;16)
Associations
Multiple Myeloma
Reflex Testing
F MM will be auto reflexed to F FGFR3 t(4;14) and F IGH MAF t(14;16) when IGH is rearranged with a locus other than BCL1
CPT Codes
88374 x6
Turnaround Time
5
Specimen Requirements
5mL peripheral blood in sodium heparin 3mL bone marrow in sodium heparin Fixed cytogenetically prepared cells in sterile centrifuge tube with pellet visible in 3:1, Methanol:Acetic Acid
Specimen Stability
Blood and bone marrow = 4°C to 25°C, specimens are stable up to 72 hours Fixed cell pellets are stable for years when stored at -28°C to -15°C
Storage & Handling
4°C to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.
Causes for Rejections
Clotted specimen; Specimens exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells
Description
Cytogenetic studies using chromosome analysis and interphase fluorescence in situ hybridization (FISH) are routine procedures for prognostic stratification of plasma cell myeloma. Several cytogenetic abnormalities such as t(4;14), del(17/17p), t(14;16), n
References
- Palumbo A, Herve A, Stefania O, et al. Revised International Staging System for Multiple Myeloma: A Report From International Myeloma Working Group. J Clin Oncol September 2015 33:2863-2869.
- Ross F, Herve A, Ameye G, et al. Report from the European Myeloma Network on interphase FISH in multiple myeloma and related disorders. Haematologica August 2017 97:1272-1277.
