Test Name
MLL(KMT2A) 11q23 Gene Rearrangement
Test Code
F MLL
Synonyms
Mixed lineage leukemia, 11q23
Associations
Acute myelogenous leukemia (AML), Acute lymphoblastic leukemia (ALL), HOX gene
CPT Codes
88374
Turnaround Time
5
Specimen Requirements
5mL peripheral blood in sodium heparin 3mL bone marrow in sodium heparin Fixed cytogenetically prepared cells in sterile centrifuge tube with pellet visible in 3:1, Methanol:Acetic Acid
Specimen Stability
Blood and bone marrow = 4°C to 25°C, specimens are stable up to 72 hours Fixed cell pellets are stable for years when stored at -28°C to -15°C
Storage & Handling
4°C to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.
Causes for Rejections
Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells
Description
Mixed lineage leukemia (MLL) abnormalities are frequently found in infant leukemias and therapy-related leukemias. The MLL gene encodes a DNA-binding protein that methylates histone H3 lysine 4 (H3K4), and positively regulates gene expression including mu
References
- Faber J et al. (2007). Klin Padiatr. Nov-Dec; 219(6):306-11.
- Krivtsov AV et al. (2007). Nat Rev Cancer. Nov; 7(11):823-33.
