Test Name
Chronic Lymphocytic Leukemia (CLL) Panel
Test Code
F CLL
Synonyms
MYB (6q23), ATM (11q22.3), +12, DLEU1 (13q14.2), TP53 (17p13)
Associations
Chronic Lymphocytic Leukemia(CLL)
The Vysis CLL Fish probe kit is indicated for detecting deletion of the LSI TP53 probe target (17p-) as an aid in identifying those patients with CLL for whom treatment with VENCLEXTA® (venetoclax) is indi
CPT Codes
88374 x4
Turnaround Time
5
Specimen Requirements
5mL peripheral blood in sodium heparin 3mL bone marrow in sodium heparin Fixed cytogenetically prepared cells in sterile centrifuge tube with pellet visible in 3:1, Methanol:Acetic Acid
Specimen Stability
Blood and bone marrow = 4°C to 25°C, specimens are stable up to 72 hours Fixed cell pellets are stable for years when stored at -28°C to -15°C
Storage & Handling
4°C to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.
Causes for Rejections
Clotted specimen; Specimens exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells
Description
Leukemias are a diverse group of diseases often characterized by multiple genetic aberrations distributed across the genome. In some cases, the same genetic aberrations are shared by different types of leukemia. Fluorescence in situ hybridization (FISH) h
References
- Emili Montserrat. (2002). Classical and new prognostic factors in chronic lymphocytic leukemia: where to now? The Hematology Journal. 3:7.
- Stilgenbauer S et al. (2002). Genetics of chronic lymphocytic leukemia: genomic aberrations and V(H) gene mutation status in pathogenesis and clinical course. Leukemia. 16(6):993-1007.
- Pettitt A.R. et al. (2001). Dysfunction in B-cell chronic lymphocytic leukemia: inactivation of ATM as an alternative to TP53 mutation. Blood. 98(3):814-822.
- Dohner et al. (2000). Chromosomal Abnormalities in Chronic Lymphocytic Leukemia. NEJM. 343:1910.
