Test Name
CBFB t(16;16), inv(16)
Test Code
F CBFB
Synonyms
inv(16)
Associations
Acute myelogenous leukemia (AML), Eosinophilia M4eo
CPT Codes
88374
Turnaround Time
5
Specimen Requirements
5mL peripheral blood in sodium heparin 3mL bone marrow in sodium heparin Fixed cytogenetically prepared cells in sterile centrifuge tube with pellet visible in 3:1, Methanol:Acetic Acid
Specimen Stability
Blood and bone marrow = 4°C to 25°C, specimens are stable up to 72 hours Fixed cell pellets are stable for years when stored at -28°C to -15°C
Storage & Handling
4°C to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.
Causes for Rejections
Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells
Description
Structural abnormalities of chromosome 16 involving the CBFB locus have been identified in a specific group of patients with acute myelomonocytic leukemia (AML) type M4 with marked eosinophilia. Inversion 16, translocation 16;16, and deletion 16q with bre
References
- Egan N et al. (2004). Deletions of CBFB in a patient with acute myelomonocytic leukemia (AML M4Eo) and inversion 16. Cancer Genet Cytogenet 154(1):60.
- Aventin A et al. (2002). Chromosome 16 inversion-associated translocations in acute myeloid leukemia elucidated using a dual-color CBFB DNA probe. Cancer Genet Cytogenet. 134(2):142.
- Huret JL. (1991). Inv(16)(p13q22), t(16;16)(p13;q22), del(16)(q22). Atlas Genet Cytogenet Oncol Haematol.
