Test Name
Burkitt/”Double Hit” Large B-cell Lymphoma Panel
Test Code
F BURKITT
Synonyms
C-MYC 8q24, BCL2 t(14;18), BCL6 3q27
Associations
"double hit" B-cell lymphoma
CPT Codes
88374 x3
Turnaround Time
5
Specimen Requirements
5mL peripheral blood in sodium heparin 3mL bone marrow in sodium heparin Fixed cytogenetically prepared cells in sterile centrifuge tube with pellet visible in 3:1, Methanol:Acetic Acid
Specimen Stability
Blood and bone marrow = 4°C to 25°C, specimens are stable up to 72 hours Fixed cell pellets are stable for years when stored at -28°C to -15°C
Storage & Handling
4°C to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.
Causes for Rejections
Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells; Improper fixative
Description
This small, multiprobe panel contributes to the distinction of Burkitt’s from large B-cell lymphoma on problematic specimens demonstrating borderline morphologic or immunophenotypic features, limited sample volume, or zero to low probability for successfu
References
- Hummel M, et al. (2006) A biologic definition of Burkitt's lymphoma from transcriptional and genomic profiling.N Engl J Med 354:2419-30.
- de Jong D. (2009) Novel lymphoid neoplasms--the borderland between diffuse large B-cell lymphoma and Burkitt's lymphoma. Haematologica 94(7): 894-896.
- Zhao X, et al. (2008) C-MYC rearrangements are frequent in aggressive mature B-Cell lymphoma with atypical morphology. Int J Clin Exp Pathol; 1:65-74.
