Test Name
BCL6 3q27 Rearrangement
Test Code
F BCL6
Synonyms
BCL6 gene; 3q27 rearrangement
Associations
B-cell lymphoma, Non-Hodgkin lymphoma (NHL), Diffuse large B cell, Follicular lymphoma
CPT Codes
88374
Turnaround Time
5
Specimen Requirements
5mL peripheral blood in sodium heparin 3mL bone marrow in sodium heparin Fixed cytogenetically prepared cells in sterile centrifuge tube with pellet visible in 3:1, Methanol:Acetic Acid
Specimen Stability
Blood and bone marrow = 4°C to 25°C, specimens are stable up to 72 hours Fixed cell pellets are stable for years when stored at -28°C to -15°C
Storage & Handling
4°C to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.
Causes for Rejections
Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells; Improper fixative
Description
The B-cell lymphoma 6 (BCL6) gene is localized to 3q27. Rearrangements of 3q27 may be seen in case diffuse large B-cell lymphoma (30-40%) and follicular lymphoma (5-15%). Structural rearrangements include translocations and microdeletions, with about one-
References
- Cuneo A and Huret JL. (2000). 3q27 rearrangements in non Hodgkin lymphoma; t(3;Var)(q27;Var) in non-Hodgkin lymphoma. Atlas Genet Cytogenet Oncol Haematol. http://atlasgeneticsoncology.org/Genes/BCL6ID20.html
- Abramson JS et al. (2005). Advances in the biology and therapy of diffuse large B-cell lymphoma: moving toward a molecularly targeted approach. Blood. 106(4):1164.
