Test Name
BCL1 – IGH/CCND1 t(11;14)
Test Code
F BCL1
Synonyms
t(11;14)(q13;q32), IGH/CCND1; Cyclin D1
Associations
B-cell lymphoma, Non-Hodgkin lymphoma (NHL), Mantle cell lymphoma (MCL)
CPT Codes
88374
Turnaround Time
5
Specimen Requirements
5mL peripheral blood in sodium heparin 3mL bone marrow in sodium heparin Fixed cytogenetically prepared cells in sterile centrifuge tube with pellet visible in 3:1, Methanol:Acetic Acid
Specimen Stability
Blood and bone marrow = 4°C to 25°C, specimens are stable up to 72 hours Fixed cell pellets are stable for years when stored at -28°C to -15°C
Storage & Handling
4°C to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.
Causes for Rejections
Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells; Improper fixative
Description
Mantle cell lymphoma can be difficult to distinguish morphologically from other B-cell malignant lymphomas. The translocation probe is designed to detect the juxtaposition of the IGH locus (14q32) next to the CCND1 (cyclin D1) gene (11q13) in both metapha
References
- Gu et al. (2004). Evaluation of peripheral blood involvement of mantle cell lymphoma by fluorescence in situ hybridization in comparison with immunophenotypic and morphologic findings. Mod Pathol. 17(5):553.
- Sun et al. (2003). Fluorescence in situ hybridization: method of choice for a definitive diagnosis of mantle cell lymphoma. Am J Hematol. 74(1):78.
- Li et al. (1999). Detection of translocation t(11;14)(q13;q32) in mantle cell lymphoma by fluorescence in situ hybridization. Am. J. Pathology. 154:51449.
- Kluin et al. (1997). FISH and related techniques in the diagnosis of lymphoma. Cancer Surv. 30:3.
