Test Name
Chromosome Analysis Bone Marrow
Test Code
CYTO BM
Synonyms
Chromosome analysis, cytogenetic analysis, karyotyping
Associations
Hematological malignancies
CPT Codes
By report 88237, 88264, 88280xn, 88291
Turnaround Time
7
Specimen Requirements
3 mL sodium heparin tube for bone marrow aspirate 5 mm of bone core sample in at least 4 mL tissue culture transport media (RPMI) using 10 mL sterile transport tube Fixed cytogenetically prepared cells in sterile centrifuge tube with 3:1, Methanol:Acetic Acid. Pellet must be visible for the volume part and the medium part 3:1, Methanol:Acetic Acid.
Specimen Stability
4°C to 25°C, specimens are stable up to 72 hours Fixed cell pellets are stable for years when stored at -28°C to -15°C
Storage & Handling
4°C to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.
Causes for Rejections
Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells
Description
Bone marrow is the tissue of choice for cytogenetic study of most hematologic conditions because it more accurately reflects what occurs in vivo than other sample types. In hematological malignant diseases, the recognized recurring chromosomal abnormaliti
References
- Dunn, B. (2005). The Cytogenetic Symposia. 2nd edition, 13-1.
- Heim et al. (1995). Cancer Cytogenetics. New York: Wiley-Liss.
- Barch, M. (1991). ACT Cytogenetic Laboratory Manual. 2nd edition, 395-396.
