Test Name
IGH/MYC t(8;14)*, paraffin
Test Code
FP IGH MYC
Synonyms
t(8;14)(q32;q24), MYC
Associations
Burkitt lymphoma
CPT Codes
88377, 88271
Turnaround Time
5
Specimen Requirements
FFPE tissue is acceptable for FISH analysis. Preferred fixative is 10% neutral buffered formalin. Tissues preserved in B5 fixative or decalcified are usually not suitable for FISH. Tumor sections cut 3-5 µm thick and mounted on positively charged organosilane coated (silanized) slides work well. Request several unstained sections (two for each probe) and one H&E stained slide
Specimen Stability
Stable indefinitely when stored at 20°C to 25°C
Storage & Handling
4°C to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.
Causes for Rejections
Improper fixative; Tissue not fixed; Insufficient tissue; Specimen exposed to extreme temperature
Description
The t(8;14)(q32;q24) joins the cellular oncogenes C-MYC with sequences from immunoglobulin heavy chain gene enhancers leading to deregulated expression of C-MYC protein. This abnormality is characteristic of Burkitt lymphoma and may be seen with other chr
References
- Janz S. DNA Repair (Amst). 2006 Sep 8;5(9-10):1213-24.
- Pienkowska-Grela et al (2005). Cancer Genet Cytogenet 156(2):114.
