Test Name
BCL6 3q27 Rearrangement, paraffin
Test Code
FP BCL6
Synonyms
BCL6 gene; 3q27 rearrangement
Associations
B-cell lymphoma, Non-Hodgkin lymphoma (NHL), Diffuse large B cell, Follicular lymphoma
CPT Codes
88377, 88271
Turnaround Time
5
Specimen Requirements
FFPE tissue is acceptable for FISH analysis. Preferred fixative is 10% neutral buffered formalin. Tissues preserved in B5 fixative or decalcified are usually not suitable for FISH. Tumor sections cut 3-5 µm thick and mounted on positively charged organosilane coated (silanized) slides work well. Request several unstained sections (two for each probe) and one H&E stained slide
Specimen Stability
Stable indefinitely when stored at 20°C to 25°C
Storage & Handling
4°C to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.
Causes for Rejections
Improper fixative; Tissue not fixed; Insufficient tissue; Specimen exposed to extreme temperature
Description
The B-cell lymphoma 6 (BCL6) gene is localized to 3q27. Rearrangements of 3q27 may be seen in case diffuse large B-cell lymphoma (30-40%) and follicular lymphoma (5-15%). Structural rearrangements include translocations and microdeletions, with about one-
References
- Cuneo A and Huret JL. (2000). 3q27 rearrangements in non Hodgkin lymphoma; t(3;Var)(q27;Var) in non-Hodgkin lymphoma. Atlas Genet Cytogenet Oncol Haematol. http://atlasgeneticsoncology.org/Genes/BCL6ID20.html
- Abramson JS et al. (2005). Advances in the biology and therapy of diffuse large B-cell lymphoma: moving toward a molecularly targeted approach. Blood. 106(4):1164.
