Test Name
Oligodendroglioma, 1p-, 19q- *, paraffin
Test Code
FP GLI
Synonyms
1p36-/19q13-, del(1)(p36), del(19)(q13)
Associations
Oligodendroglioma
CPT Codes
88377, 88271
Turnaround Time
5
Specimen Requirements
FFPE tissue is acceptable for FISH analysis. Preferred fixative is 10% neutral buffered formalin. Tissues preserved in B5 fixative or decalcified are usually not suitable for FISH. Tumor sections cut 3-5 µm thick and mounted on positively charged organosilane coated (silanized) slides work well. Request several unstained sections (two for each probe) and one H&E stained slide
Specimen Stability
Stable indefinitely when stored at 20°C to 25°C
Storage & Handling
4°C to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.
Causes for Rejections
Improper fixative; Tissue not fixed; Insufficient tissue; Specimen exposed to extreme temperature
Description
Deletions of chromosome 1p and 19q arms are frequent genetic abnormalities in primary human gliomas and are especially common in oligodendrogliomas. While 1p deletions are relatively common in other types of tumors, 19q deletions appear to be common only
References
- Law, Mark et al. (2006). Cancer Genetics and Cytogenetics. 160:1–14.
- Barbashina V et al. (2005). Allelic loss at 1p36 and 19q13 in gliomas: correlation with histologic classification, definition of a 150-kb minimal deleted region at 1p36, and evaluation of CAMTA1 as a candidate tumor suppressor gene. Clin Cancer Research. 11:1119.
- Smith JS et al. (2000). Alterations in chromosome arms 1p and 19q as predictors of survival in oligodendrogliomas, astrocytomas, and mixed oligoastrocytomas. J Clin Oncol. 18(3):636.
