Test Name
ROS1 6q22.1 rearrangement by FISH
Test Code
FP ROS1
Synonyms
6q22.1 rearrangement; Receptor Tyrosine Kinase (RTK); Non small cell lung cancer
Associations
ROS1 is a receptor tyrosine kinase (RTK) of the insulin receptor family. Chromosomal rearrangements involving the ROS1 gene, on chromosome 6q22, were originally described in glioblastomas (e.g., FIG-ROS1). More recently, ROS1 fusions were identified a
CPT Codes
88377, 88374, 88271
Turnaround Time
5
Specimen Requirements
10% neutral buffered formalin fixed paraffin embedded tissue 5 mm^3 fresh tissue or 3.0 mL (min. 2.0 mL) FNA in MPLN RPMI media
Specimen Stability
Paraffin blocks stable at 18-25°C indefinitely Fresh tissue or FNA stable at 2-8°C for 72 hours
Storage & Handling
Fresh tissue, FNA or paraffin embedded tissue, ship in a Styrofoam container with a cool/refrigerated pack (Do not allow cool pack to directly contact sample)
Causes for Rejections
Specimen exposed to extreme temperature; Insufficient specimen; Improper fixative
Description
The LSI dual break-apart rearrangement probe contains two differently labelled probes on either sides of the breakpoint of the ROS1 gene. ROS1 has multiple fusion partners including but not limited to CCDC6, CD74, EZR, KDELR2, LRIG3, SDC4, SLC34A2, and TP
References
- Lovly, C., L. Horn, W. Pao. (2015) ROS1 in Non-Small Cell Lung Cancer (NSCLC). My Cancer Genome https://www.mycancergenome.org/content/disease/lung-cancer/ros1/ (Updated November 17).
- Shaw, A,T,, Ou, S.H., et. al.(2014) Crizotinib in ROS1-Rearranged Non-Small_Cell Lung Cancer. The New England Journal of Medicine. 2014 Nov. 371 (21): 1963-1971
- Bubendorf, L., Büttner, R., Al-Dayel, F. et al. Testing for ROS1 in non-small cell lung cancer: a review with recommendations. Virchows Arch (2016) 469: 489
- Shaw, A.T., Camidge, D.R., et. al. (2012). Clinical activity of crizotinib in advanced non-small cell lung cancer (NSCLC) harboring ROS1 gene rearrangement. Journal of Clinical Oncology 30, 7508-7508
- Kurtis D. Davies, Anh T. Le, et. al. (2012). Identifying and Targeting ROS1 Gene Fusions in Non–Small Cell Lung Cancer. Clin Cancer Res September (18) (17) 4570-4579
