Test Name
SYT 18q11.2 Synovial Sarcoma*
Test Code
F SYT
Synonyms
SYT gene rearrangement, 18q11, 18q11.2 rearrangement
Associations
Synovial sarcomas
CPT Codes
88374
Turnaround Time
9
Specimen Requirements
FFPE tissue is acceptable for FISH analysis. Preferred fixative is 10% neutral buffered formalin. Tissues preserved in B5 fixative or decalcified are usually not suitable for FISH. Tumor sections cut 3-5 µm thick and mounted on positively charged organosilane coated (silanized) slides work well. Request several unstained sections (two for each probe) and one H&E stained slide
Specimen Stability
Stable indefinitely when stored at 20°C to 25°C
Storage & Handling
4°C to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.
Causes for Rejections
Improper fixative; Tissue not fixed; Insufficient tissue; Specimen exposed to extreme temperature
Description
Synovial sarcomas (SS), which account for 5-10% of soft tissue sarcomas, are seen mainly in adolescents and adults aged between 15 and 45 years. More than 85-95% of them arise in the deep soft tissues of the extremities. Synovial sarcomas harbor the t(X;1
References
- Subramaniam, Manish Mani et al. (2007). Cancer Genetics and Cytogenetics. 172:23-28.
