Test Name
Trisomy 12
Test Code
F T12
Synonyms
+12, CEP 12
Associations
Chronic Lymphocytic Leukemia (CLL)
CPT Codes
88374
Turnaround Time
5
Specimen Requirements
5mL peripheral blood in sodium heparin 3mL bone marrow in sodium heparin Fixed cytogenetically prepared cells in sterile centrifuge tube with pellet visible in 3:1, Methanol:Acetic Acid
Specimen Stability
Blood and bone marrow = 4°C to 25°C, specimens are stable up to 72 hours Fixed cell pellets are stable for years when stored at -28°C to -15°C
Storage & Handling
4°C to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.
Causes for Rejections
Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells
Description
Trisomy 12 is among the more common recurring chromosomal defects in chronic lymphocytic leukemia (CLL). It has been reported in 15–30% of cases and is correlated with atypical morphology. FISH can detect this trisomy in either interphase or metaphase cel
References
- Athanasiadou, Anastasia et al. (2006). Cancer Genetics and Cytogenetics. 168:109-119.
