Test Name
Trisomy 8
Test Code
F T8
Synonyms
+8, CEP 8
Associations
Acute myeloid leukemia (AML), Chronic myelogenous leukemia (CML), Myelodysplastic syndrome (MDS)
CPT Codes
88374
Turnaround Time
5
Specimen Requirements
5mL peripheral blood in sodium heparin 3mL bone marrow in sodium heparin Fixed cytogenetically prepared cells in sterile centrifuge tube with pellet visible in 3:1, Methanol:Acetic Acid
Specimen Stability
Blood and bone marrow = 4°C to 25°C, specimens are stable up to 72 hours Fixed cell pellets are stable for years when stored at -28°C to -15°C
Storage & Handling
4°C to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.
Causes for Rejections
Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells
Description
Trisomy 8 is relatively specific for myeloid disorders and is rarely observed in lymphoid disease. It is found in 10-15% of patients with acute myeloid leukemia (AML), 15-20% of patients with myelodysplastic syndromes (MDS), as a secondary abnormality in
References
- Matsuoka H et al. (2005). Establishment of a human myeloid cell line with trisomy 8 derived from overt leukemia following myelodysplastic syndrome.
- Wolman SR. (2002). Impact of trisomy 8 (+8) on clinical presentation, treatment response, and survival in acute myeloid leukemia: a Southwest Oncology Group study. Blood. 100(1):29.
- Huret JL.(November 1998) +8 or trisomy 8. Atlas Genet Cytogenet Oncol Haematol.
