Test Name
TP53 17p13 deletion
Test Code
F P53
Synonyms
p53, 17p13-, del(17)(p13,1)
Associations
Chronic lymphocytic leukemia (CLL), B-cell prolymphocytic leukemia (PLL), B-cell lymphoproliferative disorder (LPD), Myeloma
CPT Codes
88374
Turnaround Time
5
Specimen Requirements
5mL peripheral blood in sodium heparin 3mL bone marrow in sodium heparin Fixed cytogenetically prepared cells in sterile centrifuge tube with pellet visible in 3:1, Methanol:Acetic Acid
Specimen Stability
Blood and bone marrow = 4°C to 25°C, specimens are stable up to 72 hours Fixed cell pellets are stable for years when stored at -28°C to -15°C
Storage & Handling
4°C to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.
Causes for Rejections
Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells
Description
The p53 tumor suppressor gene is one of the most frequently mutated genes in human cancer with mutations and deletions identified in a wide range of solid tumors and hematological disorders. In hematological disease, p53 gene alterations have been found i
References
- Liu et al. (2002). P53 mutations: case study of a clinical marker for solid tumors. Semin Oncol. 29(3):246.
- Pratt G. (2002). Molecular aspects of multiple myeloma. J Clin Pathol: Mol Pathol. 55:273.
- Soussi T . (2002) P53 (Protein 53 kDa); TP53 (tumor protein p53 (Li-Fraumeni syndrome)). Atlas Genet Cytogenet Oncol Haematol.
