Test Name
IGH/MAF t(14;16)
Test Code
F IGH MAF
Synonyms
t(14;16)(q32;q23), Immunoglobulin heavy chain / MAF gene
Associations
Myeloma, MGUS
CPT Codes
88374
Turnaround Time
5
Specimen Requirements
5mL peripheral blood in sodium heparin 3mL bone marrow in sodium heparin Fixed cytogenetically prepared cells in sterile centrifuge tube with pellet visible in 3:1, Methanol:Acetic Acid
Specimen Stability
Blood and bone marrow = 4°C to 25°C, specimens are stable up to 72 hours Fixed cell pellets are stable for years when stored at -28°C to -15°C
Storage & Handling
4°C to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.
Causes for Rejections
Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells
Description
The t(14;16)(q32;q23) is detectable in 2–10% of patients with plasma cell myeloma and in about 25% of myeloma cell lines. The t(14;16)(q32;q23) is difficult to detect by conventional karyotypes. Although some studies report a low prevalence of the t(14;16
References
- Fonseca et al. (2004). Genetics and cytogenetics of multiple myeloma: A workshop report. Cancer Res. 64:1546.
- Hideshima et al. (2004). Advances in biology of multiple myeloma: clinical applications. Blood. 104:607.
- Pratt G. (2002). Molecular aspects of multiple myeloma. J Clin Pathol: Mol Pathol. 55:273.
