Test Name
IGH 14q32 Rearrangement*
Test Code
F IGH
Synonyms
Immunoglobulin heavy chain, 14q32 rearrangement
Associations
Non-Hodgkin lymphoma, Myeloma
CPT Codes
88374
Turnaround Time
5
Specimen Requirements
5mL peripheral blood in sodium heparin 3mL bone marrow in sodium heparin Fixed cytogenetically prepared cells in sterile centrifuge tube with pellet visible in 3:1, Methanol:Acetic Acid
Specimen Stability
Blood and bone marrow = 4°C to 25°C, specimens are stable up to 72 hours Fixed cell pellets are stable for years when stored at -28°C to -15°C
Storage & Handling
4°C to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.
Causes for Rejections
Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells; Improper fixative
Description
Rearrangements involving the IGH gene have been identified in about 50% of non-Hodgkin B-cell lymphomas (NHLs) and correlated to clinically relevant subgroups. A t(14;18)(q32;q21) is found in 88.1% of follicular lymphomas and a t(11;14)(q13;q32) is in 85.
References
- Bernicot I et al. (2007). Cytogenet Genome Res. 118:345-352.
- Letter to the editor. (2007). Cancer Genetics and Cytogenetics. 172:172-173.
- Peinkowska-Grela et al. (2005). Frequent aberrations of chromosome 8 in aggressive B-cell non-Hodgkin lymphoma. Cancer Genet Cytogenet. 156(2):114.
