Test Name
ZAP 70
Test Code
FLOW ZAP 70
Synonyms
ZAP-70
Associations
Chronic lymphocytic leukemia(CLL); IgVH mutational status; TP53 mutation status
Reflex Testing
None
CPT Codes
88184, 88185x3, 88187
Turnaround Time
1
Specimen Requirements
Whole blood: 4 mL (min 1 mL) in sodium heparin or EDTA; Bone marrow aspirate: 2 mL (min 0.5 mL) in sodium heparin or EDTA
Specimen Stability
Specimen stable for 48 hours at 4°C
Storage & Handling
Ship with ice pack. Protect from extreme temperature. Separate ice pack from specimen.
Causes for Rejections
Specimen >48 hours old prior to testing; Specimens stored at incorrect temperature; Non-viable specimens; Specimens in inappropriate anticoagulant; Too few cells; Hemolysis; Specimen clotted
Description
ZAP-70 testing by Flow cytometry
References
- Shanafelt (2009). B-cell count and Survival: differentiating CLL from monoclonal B-cell lymphocytosis based on clinical outcome. Blood. 113, No.18: 4188-4196.
- Craig and Foon. (2008). Flow Cytometric Immunophenotyping for Hematologic Neoplasms, Blood. 111, No.8: 3941-3967.
- Rawstron et al (2008). Monoclonal B-cell lymphocytosis and CLL. NEJM. 359;6:575-583.
- Special Issue. (2006). Zap-70 and CLL. Cytometry, 70B:4.
- Shanafelt et al. (2004). Prognosis at diagnosis: integrating molecular biologic insights into clinical practice for patients with CLL. Blood. 103(4):1202.
- Crespo et al. (2003). ZAP-70 expression as a surrogate for Immunoglobulin-Variable-Region mutations in chronic lymphocytic leukemia. NEJM. 348(18):1764.
