Test Name
High Sensitivity PNH (FLAER)
Test Code
FLOW PNH
Synonyms
PNH Screen, high sensitivity analysis for PNH, CD59, FLAER, GPI deficiency
Associations
Coombs negative hemolytic anemia, myelodyspastic syndromes with unilineage dysplasia and unexplained hemoglobinuria, aplastic anemia, cytopenia or thrombosis;
Soliris® (Eculizumab)
Reflex Testing
None
CPT Codes
88184, 88185x6, 88187
Turnaround Time
1
Specimen Requirements
Whole blood: 5 mL (min. 0.5 mL) in sodium heparin or EDTA
Specimen Stability
Specimen stable for 48 hours at 4°C
Storage & Handling
Ship with ice pack. Protect from extreme temperature. Separate ice pack from specimen.
Causes for Rejections
Specimen >48 hours old prior to testing; Specimens stored at incorrect temperature; Non-viable specimens; Specimens in inappropriate anticoagulant; Too few cells; Excessive hemolysis; Specimen clotted
Description
High sensitvity PNH testing by Flow cytometry
References
- Dezern AE, Borowitz MJ 2018, ICCS/ESCCA consensus guidelines to detect GPI-deficient cells in paroxysmal nocturnal hemoglobinuria (PNH) and related disorders part 1 - clinical utility. Cytometry 2018 Jan;94(1):16-22.
- Southerland et al. (2007). Diagnosing PNH with FLAER and multiparameter flow cytometry. Cytometry. 72B:167–177.
- Parker C. et al. Diagnosis and management of paroxysmal nocturnal hemoglobinuria. Blood. 106: 3699-3709 2005
- Richards SJ et al. Recent advances in the diagnosis monitoring and management of patients with paroxysmal nocturnal hemoglobinuria. Cytometry. 72B: 291-298 2007
