T-cell subsets, CD4:CD8 ratio and count


Test Name

T-cell subsets, CD4:CD8 ratio and count

Test Code

FLOW CD4*

Synonyms

CD4/CD8 count; T helper / T suppressor analysis; lymphocyte subsets

Associations

HIV, CD4

Reflex Testing

None

CPT Codes

86359, 86360

Turnaround Time

3

Specimen Requirements

Whole blood: 4 mL (min. 0.5mL) in EDTA (Lavender), K EDTA (pink), or sodium or lithium heparin (green)

Specimen Stability

Specimen stable for 72 hours at room temperature in EDTA; 48 hours at room temperature in heparin

Storage & Handling

Ship ambient. Protect from extreme temperature with an ice pack. Separate ice pack from specimen.

Causes for Rejections

Clotted or hemolyzed specimens. Refrigerated: Unacceptable; Frozen: Unacceptable.

Description

CD3,CD4,CD8,CD4:CD8 by Flow cytometry

References

  1. Maurice R. G. O'Gorman and Janet K. A. Nicholson. (2000). Adoption of single-platform technologies for enumeration of absolute T-lymphocyte subsets in peripheral blood. Clin Diagn Lab Immunol. 7(3):333–335.

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