Test Name
BCL2 t(14;18) Gene Rearrangement
Test Code
M BCL2
Synonyms
JH gene rearrangement
Associations
B-cell lymphoma, Follicular lymphoma, Diffuse large cell lymphoma, Mantle lymphoma, MALT lymphoma
CPT Codes
81402
Turnaround Time
3-5 days
Specimen Requirements
- 5.0 mL (min. 3.0 mL) whole blood EDTA or Sodium Heparin
- 3.0 mL (min. 1.0 mL) bone marrow EDTA or Sodium Heparin
- 5 mm^3 bone marrow core biopsy, fresh or frozen tissue in MPLN RPMI
- 3.0 mL (min. 2.0 mL) FNA in MPLN RPMI medium
- Formalin fixed paraffin embedded tissue
Specimen Stability
EDTA whole blood or bone marrow stable at 18-25°C for 72 hours Bone marrow biopsy, fresh tissue or FNA in MPLN RPMI at stable at 2-8°C for 72 hours Frozen tissue at -20°C stable indefinitely Paraffin-embedded tissue at 18-25°C stable indefinitely
Storage & Handling
Whole blood, bone marrow, bone marrow biopsy, fresh tissue or FNA, ship in a Styrofoam container with a cool/refrigerated pack (Do not allow the cool pack to directly contact the sample.) Frozen tissue, ship on dry ice Paraffin embedded tissue ship ambien
Causes for Rejections
Insufficient volume; Specimen at incorrect temperature; Incorrect anticoagulant or fixative
Description
by PCR
References
- Weinberg OK et al. (2007). “Minor” BCL2 breakpoints in follicular lymphoma. Frequency and correlation with grade and disease presentation in 236 cases. J Molec Diagn. 9:530-537.
- Van Dongen JJM et al. (2003). Design and standardization of PCR primers and protocols for detection and clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: Report of the BIOMED-2 Concerned Action BMH4-CT98-3936. Leukemia. 17:2257-2317.
