Test Name
T-cell Receptor Gamma Gene Rearrangement
Test Code
M TCR
Synonyms
T-cell receptor gamma (TCRγ) gene rearrangement, T cell gene rearrangement
CPT Codes
83891, 83900, 83901x18, 83909, 83912-26, 83907
Turnaround Time
7 days
Specimen Requirements
5.0 mL (min. 3.0 mL) whole blood EDTA or Sodium Heparin 3.0 mL (min. 1.0 mL) bone marrow EDTA or Sodium Heparin 5 mm^3 bone marrow core biopsy, fresh tissue or frozen tissue in MPLN RPMI 3.0 mL (min. 2.0 mL) FNA in MPLN RPMI medium Formalin fixed paraffin embedded tissue
Specimen Stability
Whole blood or bone marrow stable at 18-25°C for 72 hours Bone marrow biopsy, fresh tissue or FNA in MPLN RPMI stable at 2-8°C for 72 hours Frozen tissue stable at -20°C indefinitely Paraffin embedded tissue stable at 18-25°C indefinitely
Storage & Handling
Whole blood, bone marrow, bone marrow biopsy, fresh tissue or FNA, ship in a Styrofoam container with a cool/refrigerated pack (Do not allow the cool pack to directly contact the sample.) Frozen tissue, ship on dry ice Paraffin embedded tissue, ship ambie
Causes for Rejections
Improper specimen labeling; Insufficient sample volume; Clotted specimen; Specimen older than 7 days
Description
Molecular characterization of T-cell receptor gene rearrangements and immunoglobulin heavy and light chain gene rearrangement detection is accomplished with reliable and robust tests to identify clonal T-cell and B-cell populations in a variety of cutaneo
References
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- Giudicelli, V., Chaume, D., & Lefranc, M.-P. (2005). IMGT/GENE-DB: a comprehensive database for human and mouse immunoglobulin and T cell receptor genes. Nucleic Acids Research, 33(suppl 1), D256-D261. doi:10.1093/nar/gki010
- Invivoscribe. (2017). LymphoTrack TRG Assay -MiSeq. 280323 Rev. N - Feb. 2017.
- Lymphoma Research Foundation. (2017, June 07). Retrieved from www.lymphoma.org/Tcell: www.lymphoma.org/Tcell
- Pimpinelli, N., Olsen, E. A., Santucci, M., Vonderheid, E., Haeffner, A. C., Stevens, S., . . . Sa. (2017). Defining early mycosis fungoides. Journal of the American Academy of Dermatology, 53(6), 1053-1063. doi:10.1016/j.jaad.2005.08.057
- Schumacher, J. A., Duncavage, E. J., Mosbruger, T. L., Szankasi, P. M., & Kelley, T. W. (2014). A Comparison of Deep Sequencing of TCRG Rearrangements vs Traditional Capillary Electrophoresis for Assessment of Clonality in T-Cell Lymphoproliferative Disorders. American Journal of Clinical Pathology, 141(3), 348-359. doi:10.1309/AJCP5TYGBVW4ZITR
- Sufficool, K. E., Lockwood, C. M., Abel, H. J., Hagemann, I. S., Schumacher, J. A., Kelley, T. W., & Duncavage, E. J. (2015). T-cell clonality assessment by next-generation sequencing improves detection sensitivity in mycosis fungoides. Journal of the American Academy of Dermatology, 73(2), 228-236.e2.
- van Dongen, J., Langerak, A., Brüggemann, M., Evans, P., Hummel, M., Lavender, F., . . . Macintyre, E. (2003). Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: Report of the BIOMED-2 Concerted Action BMH4-CT98-3936. Leukemia, 17, 2257-2317.
- Wilcox, R. A. (2014). Cutaneous T-cell lymphoma: 2014 Update on diagnosis, risk-stratification, and management. American Journal of Hematology, 89(8), 837-851. doi:10.1002/ajh.23756
- Willemze, R., Jaffe, E. S., Burg, G., Cerroni, L., Berti, E., Swerdlow, S. H., . . . Kurrer, M. (2005). WHO-EORTC classification for cutaneous lymphomas. Blood, 105(10), 3768-3785. doi:10.1182/blood-2004-09-3502
