Herpes SimplexVirus Type 2

Test Name

Test Code

HSV2

Associations

Detection of herpes simplex virus (HSV) by polymerase chain reaction (PCR), has proven to be the most specific, rapid and sensitive means to diagnose anogenital and central nervous system infections. Studies show the range of sensitivity of PCR detect

CPT Codes

87529

Turnaround Time

1-3 days

Specimen Requirements

HSV lesion MPLN path. collection kit
2.0 mL whole blood EDTA
5 mm^3 fresh or frozen tissue
Swab from any site MPLN pathogen collection kit or viral transport media
2.0 mL (min. 1.0 mL) CSF sterile container, DO NOT DILUTE
2.0 mL (min. 1.0 mL) Cervical cells, ThinPrep® PAP solution or SurePath™ Test Pack solution
1.0 mL amniotic fluid, sterile container, unspun

Specimen Stability

MPLN pathology collection kit, 18-25°C, ambient
Whole blood, 4°C, stable 96 hours
Fresh tissue, 4°C, stable 96 hours: Frozen tissue, -20°C, freeze immediately
Swab from any site, 4°C, stable 96 hours

Storage & Handling

MPLN pathology collection kit, ship ambient
Whole blood, ship with cool/refrigerated pack. Separate cool/refrigerated pack from specimen
Fresh Tissue, MPLN transport medium or equivalent (i.e. RPMI, Hanks, M

Causes for Rejections

Improper anticoagulant; Improper shipping media; Recommended temperatures exceeded; Age of specimen exceeded

Description

Intended for use to assist in the confirmation of a diagnosis of a HSV-1 and/or HSV-2 infection.

References

Salvaggio MR (2009). Herpes Simplex.Medscape.
Kriebs JM. (2008). Understanding herpes simplex virus: Transmission, diagnosis, and considerations in pregnancy management. J Midwifery Womens Health. 53:202–208.

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